Journal: Journal of Biological Chemistry

Article Title: Chaperone Insufficiency Links TLR4 Protein Signaling to Endoplasmic Reticulum Stress

doi: 10.1074/jbc.m111.315218

Figure Lengend Snippet: FIGURE 6. GRP94 and GRP78 control ERS in THP-1 cells. In A–C, THP-1 cells were maintained in steady state (CTR) or treated with LPS (24 h) or submitted to glucose deprivation without LPS treatment (GLU-R) or submitted to glucose deprivation followed by LPS treatment (GLU-R24 h LPS). Protein extracts were separated by SDS-PAGE and immunoblotted with anti-spliced XBP1 (A), peIF2 (B), or ATF6 (C) antibodies. In D and E, THP-1 cells were treated with Lipo- fectaminealone(Lipo)orinthepresenceofglucosedeprivation(LipoGlu-R)orwithacontrolsiRNA(siCTR),aGRP94(D),oraGRP78(E)siRNA(siGRP94orsiGRP78, respectively), or a GRP94 (D) or a GRP78 (E) siRNA in the presence of glucose deprivation (siGRP94Glu-R or siGRP78Glu-R, respectively). Protein extracts were separated by SDS-PAGE and immunoblotted with anti-GRP94 (D) or GRP78 (E) antibodies. In F–L, THP-1 cells were treated with Lipofectamine alone (Lipo) or in the presence of LPS, glucose deprivation (LipoGlu-R), glucose deprivation plus LPS (GLU-RLPS) in the absence of presence of a GRP94 (F–H and L), or a GRP78 (I–K) siRNA (siGRP94 or siGRP78, respectively). Protein extracts were separated by SDS-PAGE and immunoblotted with anti-spliced XBP1 (F and I), pPERK (G and J), or ATF6 (H and K) antibodies or real-time PCR was used to determine the expression of IL-8 (L). In A–K, all membranes were stripped and reblotted with anti--actin. In all experiments, n 6. *, p 0.05 versus CTR in A–C or versus Lipofectamine alone in D–L. In A and B, #, p 0.05 versus 24-h LPS. asu, arbitrary scanning units.

Article Snippet: In some experiments, cells were deprived of glucose for 6 h or treated with siRNA (GRP94 siRNA, sc35523 and GRP78 siRNA, sc29338 from Santa Cruz Biotechnology) to increase or decrease, respectively, the expression of the chaperones.

Techniques: Control, SDS Page, Real-time Polymerase Chain Reaction, Expressing

Journal: Thoracic Cancer

Article Title: Overexpression of molecule GRP94 favors tumor progression in lung adenocarcinoma by interaction with regulatory T cells

doi: 10.1111/1759-7714.13321

Figure Lengend Snippet: GPR94 expression in lung AD and the interaction with FOXP3. ( a ) GRP94 protein levels in 33 lung AD tissue and adjacent normal lung tissue (ANT) samples by IHC. ( b ) GRP94 was overexpressed in lung AD tissue compared with ANT samples ( P < 0.05). ( c ) The expression level of FOXP3 in lung AD tissue indicated FOXP3 + Tregs infiltrated into the tumor microenvironment.

Article Snippet: The small interfering RNA (siRNA) for GRP94 was provided by Ribobio (Guangzhou, China).

Techniques: Expressing

Journal: Thoracic Cancer

Article Title: Overexpression of molecule GRP94 favors tumor progression in lung adenocarcinoma by interaction with regulatory T cells

doi: 10.1111/1759-7714.13321

Figure Lengend Snippet: Ggp94 expression in 80 lung AD patients

Article Snippet: The small interfering RNA (siRNA) for GRP94 was provided by Ribobio (Guangzhou, China).

Techniques: Expressing

Journal: Thoracic Cancer

Article Title: Overexpression of molecule GRP94 favors tumor progression in lung adenocarcinoma by interaction with regulatory T cells

doi: 10.1111/1759-7714.13321

Figure Lengend Snippet: GPR94 expression in lung AD predicts overall survival. ( a ) Lung AD patients with high levels of GRP94 had a significantly shorter overall survival than those with low levels of GRP94 (hazard ratio [HR] = 2.12 [95% CI 1.23–3.04]; P = 0.007) ( ) Hi and ( ) Lo. ( b ) Lung AD patients with a high FOXP3 + Treg count had a significantly shorter overall survival (HR = 1.73 [95% CI 1.09–2.75]; P = 0.016) ( ) Hi and ( ) Lo.

Article Snippet: The small interfering RNA (siRNA) for GRP94 was provided by Ribobio (Guangzhou, China).

Techniques: Expressing

Journal: Thoracic Cancer

Article Title: Overexpression of molecule GRP94 favors tumor progression in lung adenocarcinoma by interaction with regulatory T cells

doi: 10.1111/1759-7714.13321

Figure Lengend Snippet: Grp94 expression interaction with Foxp3+ Tregs

Article Snippet: The small interfering RNA (siRNA) for GRP94 was provided by Ribobio (Guangzhou, China).

Techniques: Expressing

Journal: Thoracic Cancer

Article Title: Overexpression of molecule GRP94 favors tumor progression in lung adenocarcinoma by interaction with regulatory T cells

doi: 10.1111/1759-7714.13321

Figure Lengend Snippet: GRP94 promotes cell proliferation and inhibits cell apoptosis in A549 cells. ( a ) A CCK‐8 method was used to observe the effect of GRP94 on the proliferation of A549 cells. GRP94 gene knockout significantly inhibited A549 cell proliferation ( P < 0.05). ( b ) The effect of GRP94 on lung AD cell apoptosis was evaluated using flow cytometric analysis. GRP94 knockdown had a significant effect on the rate of cell apoptosis ( P < 0.01). ( c ) Western blotting was used to detect apoptosis‐related proteins. GRP94 knockout significantly increased the level of the apoptosis‐inducing proteins, CHOP and caspase‐7. The depletion of GRP94 significantly reduced levels of TGF‐β and SMAD2.

Article Snippet: The small interfering RNA (siRNA) for GRP94 was provided by Ribobio (Guangzhou, China).

Techniques: CCK-8 Assay, Gene Knockout, Knockdown, Western Blot, Knock-Out